![]() ![]() Ensure that the selected DNA polymerases are able to incorporate the modified nucleotides.Consider using an additive or co-solvent specifically formulated for a given DNA polymerase (e.g., GC Enhancer supplied with Invitrogen Platinum DNA polymerases).ĭUTP or modified nucleotides in reaction mix.Increase the amount of DNA polymerase, or use DNA polymerases with high processivity.Adjust the annealing temperatures, as high concentrations of PCR additives or co-solvents weaken primer binding to the target.Use the lowest possible concentration when appropriate. Review the recommended concentrations of PCR additives or co-solvents.For example, Pfu DNA polymerase works better with MgSO 4 than with MgCl 2. Check the DNA polymerase’s preference for magnesium salt solutions.The presence of EDTA, other metal chelators, or atypically high concentrations of dNTPs may require a higher Mg 2+ concentration. Optimize Mg 2+ concentration for maximum PCR yields.Increase the amount of DNA polymerase if the reaction mixture contains a high concentration of an additive (e.g., DMSO, formamide) or inhibitors from the sample sources.Review recommendations on the amount of DNA polymerase to use in PCR, and optimize as necessary.Alternatively, set up PCR on ice, or add DNA polymerase last to the reaction mixture.Hot-start DNA polymerases also increase yields of the desired PCR products by eliminating nonspecific amplification. Use hot-start DNA polymerases to prevent degradation of primers by the 3’→5’ exonuclease activity of proofreading DNA polymerases.For long PCR and PCR with degenerate primers, start with a minimum concentration of 0.5 μM.Optimize primer concentrations (usually in the range of 0.1–1 μM).Reconstitute fresh primer aliquots, or obtain new primers if necessary.Aliquot primers after resuspension and store properly.Verify that the primers are complementary to the correct strands of the target DNA.Ensure that the primers are specific to the target of interest.Use online primer design tools when appropriate. ![]()
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![]() ![]() This also means that you can transfer your photos / videos on the go, as long as you have a WiFi connection.įor anyone wondering how to transfer securely, the Photo Transfer - Wireless/Wifi app also offers a good solution. All you need is your smartphone and computer connected to a WiFi network. ![]() Photo Transfer - Wireless/Wifi app is the ultimate person’s solution for moving photos / videos. Download multiple photos or videos at once and start transferring your images the easy way! This app offers a simple and light solution for transferring photos and videos to your computer without losing any quality. How to transfer your photos and videos on a computer?ġ) Open the app and type the URL provided by the app in your computer’s web browserĢ) Choose photos or videos to download and save them to your PC or MAC It’s smooth and fast!įirst, check that your iPhone and computer are connected to the same Wi-Fi network, then you’re ready to start transferring! The photo transfer app uses WiFi to copy your iPhone photos and videos to your computer. Whether you want to transfer photos / videos to back them up on your computer or to share with friends, Photo Transfer - Wireless/Wifi app can get the job done in seconds. Photo Transfer - Wireless/Wifi app can copy pictures and videos from iPhone to computer using only a Wi-Fi connection. If you have ever wondered how to transfer images and videos from smartphone to computer without using a cable or cloud service, then you have come to the right place! ![]() Pictures and videos are transferred locally, they never go on the Internet. Photo Transfer - Wireless/Wifi Transfer allows you to quickly download and backup photos & videos between your iPhone and your computer (PC or MAC) wirelessly, over Wi-Fi. ![]() ![]() ![]() Which brings us to: you're not limited by the cards of the Navigator leader, be on the lookout for strong cards from any leader, with smuggling/black market/ships. ![]() But you can also grab whatever good ones you're offered from other leaders, like Land Tax from builder, Talisman/Sacred Crab/Marionettes from Shaman, etc. You'll want improved economy cards too, and there's several ways to go, some of the strongest are Auction, Tulip, Tourism, and Budgeting(Navigator tends to deck bloat somewhat, especially once ships/smuggling gets involved). Also any other leader's population growing cards. Breed, immigration, and kidnap all work, though kidnap without fishing/fishery/other ephemeral card generation will quickly deplete the cards you have to feed it, and it kinda sucks to buy cards just to feed them to kidnap(but do buy cheap cards for it if you have it, ambush for 12 gold? sold! cheap 2-3 ratizens), and at a pinch Warren(especially multiples) can be ok, also lvl2 demolition(use it to upgrade your houses). You'll want some form of population scaling too. Smuggling, fishing, market, black market, and fishery all great too, you'll need at least one of them fairly early. If you see Paddling before you see Keep, pick that up, for the same reason, but it's a stock card so you'll need some way to resupply it(besides leader ability) or it'll become a dead card pretty soon. ![]() This lets you buy and play cards at a good rate. Keep effectively increases your card draw by 4, almost doubling your card economy, and really improves your cheese and pottery cards in the early game. Handgunners are decent if you can get their stock up high, but they'll never compete with Treasure Hunter.Įarly game you NEED a new unit, the starters aren't going to cut it, Bandit is actually pretty ok early on because it also gives you economy(gives you money when it attacks) but it slows down draw, which is great if you pick up Auction for economy, not so much otherwise.īest early card to get you going is Keep. Second best is mortarrats, third is probably blackbeards but they need some form of healing or your scaled blackbeards are going to die and thus lose all the scaling. This is further improved by cards that give you more chests(treasure ship, salvage, etc). They get obscene amount of attack if you get them early(those in play scale every time you open a chest). ![]() ![]() ![]() ![]() ![]() The characters of the game, who play football in a rather unusual way, namely with their heads, are already preparing for the new football season with might and main. 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It's too bad because it is otherwise the best audio app I've found for iPad.īack to shuffling my files back and forth between iPad and a PC with Audacity - less $10.įolks, if you could add this it would really help but I see people have been asking for this from you for 5 or 10 years so I guess that is your answer. ![]() This looked close but after purchasing I now know it doesn't have the very feature I need most - background noise sample/remove.įrom a review I saw something like "Audacity for iPad" and from someone's video I saw that it had lots of filter options such as Noise Gate type filter and thought (incorrectly) it would have some sort of background noise sample and removal. I'm still looking for a replacement for Audacity on the PC so I can do all my editing on iPad. Still need Audacity on PC for background noise removal Many thanks to the developers for a an awesome tool! (Also of note: hubby is a pro sound engineer & musician & was impressed by this app's capabilities). It's stable, works fast, and easy to return to when ya haven't used it in a while. I'll work on a project for several hours straight, every few months. I'm an audio 'enthusiast', so I need more than just the basics, delivered in an intuitive format. I'm sure there are more advanced things this app doesn't do, but for my needs (fader, crop, cut/paste, pitch, speed, loop, eq, convert, etc.), TwistedWave works great! I use Document Picker to export to a variety of places, and often use the audio in slideshows or new videos. Plus, I've used it to edit single track files from my archive, as well as others people send me from other sources &/or need edited (that I pull from video, etc). I mainly use this to edit recordings made with the native iOS Voice Memo app. ![]() Send files to TwistedWave by drag and dropping them in the browser.Play and download the files directly from a browser.(30 days for free on TwistedWave's servers)Ĭonnect to the TwistedWave app from a web browser on another device or computer on the same WiFi network, and: Browser access to allow downloading and uploading files from/to TwistedWave.Export multiple files in a single ZIP file.Browser access, send a file to TwistedWave from a device on the same WiFi network.Import files from an SD card or USB drive, with the right adapter.MP3 (Encoded with the LAME MP3 encoder for the best quality).Convert the sampling rate (with SoX' very high quality resampler).Filters (low-pass, high-pass, band-pass, low-shelf, high-shelf).Amplify or normalize the audio to a given level.Peak limiter to amplify audio without causing distortion.Dynamics processor, can be used as a compressor or a noise gate.All the AudioUnit extensions (AUv3) available on the App Store can be used with TwistedWave.Precision Time Stretching & Pitch Shifting technology licensed from ZTX.Record audio from other music applications with Audiobus.Record studio quality audio with an external microphone.Record stereo audio with the built-in microphone (iPhone XS or more recent). ![]() Up to 24 bit / 96 kHz audio if your audio interface supports it.No duration limit other than the available storage.Unlimited undo / redo history, happens instantly.TwistedWave is a very fast and easy to use audio editor and recorder.Įven when working with huge files, it will remain very fast and fluid, and will never run out of memory. ![]() ![]() ![]() Read more: Best Comedy Game 2017: West of Loathing Skribbl. ![]() If you like Kingdom of Loathing's sense of humor, check out the non-browser cowboy RPG West of Loathing, by the same developers. And while you can't directly play with other people, you can steal their stuff, join guilds and interact. The whole game is consistently absurd and amusing, from the enemy types, to the genre conventions it apes so cleverly. We have over 726 of the best Multiplayer games for you Play online for free at Kongregate, including SAS: Zombie Assault 3, ShellShock Live 2, and Bad Eggs Online 2 We have reduced support for legacy browsers. And then there's the Haiku Dungeon, where not only are all the descriptions of your enemies in haiku form, but so are your attacks. The Disco Bandits dance at their enemies, fuelled by moxie. The Sauceror flings hot sauce in people's faces, and they get damaged, because hot sauce would really hurt if someone threw it at your face. Take, for instance, the pun-based classes. It's trying to be funny, and it's been going strong for years. ![]() It's a little ugly, but Kingdom of Loathing isn't trying to be pretty. Kingdom of Loathing is a sort of pseudo-mmo kind of thing, firmly embedded in the web interface, with drop down menus letting you select your attacks, and page refreshes for every new area. The only things that you need to play those games are internet. Play: Kingdom of Loathing | Release date: 2003 | Developer: Asymmetric Publications You can find free and fun browser games under this category. ![]() |
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